MCQ Question for Class 12 Biology Chapter 12 Biotechnology and its Application

Check the below NCERT MCQ Class 12 Biology Chapter 12 Biotechnology and its Application with Answers available with PDF free download. MCQ Questions for Class 12 Biology with Answers were prepared based on the latest syllabus and examination pattern issued by CBSE, NCERT and KVS. Our teachers have provided below Biotechnology and its Application Class 12 Biology MCQs Questions with answers which will help students to revise and get more marks in exams

Biotechnology and its Application Class 12 Biology MCQs Questions with Answers

Refer below for MCQ Class 12 Biology Chapter 12 Biotechnology and its Application with solutions. Solve questions and compare with the answers provided below

Question. What triggers the activation of protoxin to active toxin of Bacillus thuringiensis in boll worm?
(a) Acidic pH of stomach
(b) Body temperature
(c) Moist surface of midgut
(d) Alkaline pH of gut

Question. In RNAi, the genes are silenced using
(a) dsRNA
(b) ssDNA
(c) ssRNA
(d) dsDNA.

Question. The plasmid of which one of the following has ‘nif’ gene in it?
(a) Rhizobium
(b) Agrobacterium tumefaciens
(c) Bacillus thuringiensis
(d) Salmonella typhimurium

Question. Which one of the following vectors is used to replace the defective gene in gene therapy?
(a) Adenovirus
(b) Cosmid
(c) Ri plasmid
(d) Ti plasmid

Question. The genetic defect, adenosine deaminase (ADA) deficiency may be cured permanently by
(a) administering adenosine deaminase through injection
(b) bone marrow transplantation
(c) enzyme replacement therapy
(d) introducing isolated gene from marrow cells producing ADA into the cells at early embryonic stages

Question. The illegal and unlawful development of biomaterials without payment to the inhabitants of their origin is called
(a) biopatent
(b) biotechnology
(c) biowar
(d) biopiracy.

Question. Transgenic animals are extensively used for all of the following procedures except
(a) bioremediation
(b) chemical safety
(c) vaccine safety
(d) toxicity test.

Question. Which one of the following is not a GMO?
(a) Bt brinjal
(b) Golden rice
(c) Tracy
(d) Dolly

Question. Maximum number of existing transgenic animals is of
(a) fish
(b) mice
(c) cow
(d) pig.

Question. In India, the organisation responsible for assessing the safety of introducing genetically
modified organisms for public use is
(a) Indian Council of Medical Research (ICMR)
(b) Council for Scientific and Industrial Research (CSIR)
(c) Research Committee on Genetic Manipulation (RCGM)
(d) Genetic Engineering Approval Committee (GEAC).

Question. Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called
(a) bio-infringement
(b) biopiracy
(c) biodegradation
(d) bioexploitation.

Question. Which of the following genes do not occur naturally in living organisms?
(a) Bt genes
(b) RNAi genes
(c) Cry genes
(d) Endogenous cytoplasmic defense genes

Question. Basic principle of developing transgenic plants and animals is to introduce the gene of
interest into the nucleus of
(a) somatic cell
(b) vegetative cell
(c) germ cell
(d) body cell.

Question. Protein encoded by gene cryIAb controls the infestation of which of the following insects?
(a) Cotton bollworm
(b) Anopheles mosquito
(c) Corn borer
(d) Aedes mosquito

Question. Which of the following Bt crops is being grown in India by the farmers?
(a) Brinjal
(b) Soybean
(c) Maize
(d) Cotton

Question. Bt brinjal is an example of transgenic crops. In this, Bt refers to
(a) Bacillus tuberculosis
(b) biotechnology
(c) b-carotene
(d) Bacillus thuringiensis.

Question. Some of the characteristics of Bt cotton are
(a) long fibre and resistance to aphids
(b) medium yield, long fibre and resistance to beetle pests
(c) high yield and production of toxic protein crystals which kill dipteran pests
(d) high yield and resistance to bollworms.

Question. cryIIAb and cryIAb produce toxins that control
(a) cotton bollworms and corn borer respectively
(b) corn borer and cotton bollworms respectively
(c) tobacco budworms and nematodes respectively
(d) nematodes and tobacco budworms respectively

Question. Which kind of therapy was given in 1990 to a four-year-old girl with adenosine deaminase
(ADA) deficiency?
(a) Gene therapy
(b) Chemotherapy
(c) Immunotherapy
(d) Radiation therapy

Question. The first human hormone produced by recombinant DNA technology is
(a) insulin
(b) estrogen
(c) thyroxin
(d) progesterone.

Question. In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is   
(a) Indian Council of Medical Research (ICMR)
(b) Council for Scientific and Industrial Research (CSIR)
(c) Research Committee on Genetic Manipulation (RCGM)
(d) Genetic Engineering Appraisal Committee (GEAC).

Question. An improved variety of transgenic basmati rice     
(a) does not require chemical fertilizers and growth hormones
(b) gives high yield and is rich in vitamin A
(c) is completely resistant to all insect pests and diseases of paddy
(d) gives high yield but has no characteristic aroma.

Question. ELISA is used to detect viruses where the key reagent is   
(a) alkaline phosphatase
(b) catalase
(c) DNA probe
(d) RNase.

Question. Which of the following Bt crops is being grown in India by the farmers?
(a) Brinjal
(b) Soybean
(c) Maize
(d) Cotton

QuestionToxsin is :
(a) Intracellular lipid
(b) Intracellular crystal protein
(c) Extracellular Crystal protein
(d) Lipid

Question. Which one of the following is a correct statement?     
(a) “Bt” in “Bt-cotton” indicates that it is a genetically modified organism produced through biotechnology.
(b) Somatic hybridization involves fusion of two complete plant cells carrying desired genes.
(c) The anticoagulant ‘hirudin’ is being produced from transgenic Brassica napus seeds.
(d) “Flavr Savr” variety of tomato has enhanced the production of ethylene which improves its taste

Question. What is antisense technology?   
(a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
(b) RNA polymerase producing DNA
(c) A cell displaying a foreign antigen used for synthesis of antigens
(d) Production of somaclonal variants in tissue cultures

Question. Maximum number of existing transgenic animals is of   
(a) fish
(b) mice
(c) cow
(d) pig.

Question. Select the correct statement(s)- 
(1) IARI has released a mustard variety rich in vitamin C.
(2) Pusa Sawani variety of Okra is resistant to aphids.
(3) Hairiness of leaves provides resistance to insect pests.
(4) Agriculture accounts for approximately 33% of India’s GDP and employs nearly 62% of the population.
(a) (1) and (2)
(b) (2) and (3)
(c) (1), (3) and (4)
(d) None of these

Question. Natural genetic engineer is :   
(a) Bacillus subtilis
(b) Pseudomonas sp.
(c) Escherichia coli
(d) Agrobacterium tumefaciens

Question .Which Vitamin has been transferred to golden rice?   
(a) Vit-B₁₂
(b) Vit-A
(c) Vit-D
(d) Vit-C

Question. Silencing of mRNA has been used in producing transgenic plants resistant to   
(a) boll worms
(b) nematodes
(c) white rusts
(d) bacterial blights.

Question. Golden rice is a promising transgenic crop. When released for cultivation, it will help in     
(a) producing a petrol-like fuel from rice
(b) alleviation of vitamin A deficiency
(c) pest resistance
(d) herbicide tolerance.

Question. Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services? 
(a) Genetic Engineering Approval Committee
(b) Research Committee on Genetic Manipulation
(c) Bio-safety committee
(d) Indian Council of Agricultural Research

Question. In transgenics, expression of transgene in target tissue is determined by   
(a) enhancer
(b) transgene
(c) promoter
(d) reporter.

Question. Which one of the following is now being commercially produced by biotechnological procedures? 
(a) Nicotine
(b) Morphine
(c) Quinine
(d) Insulin

Question. Anticoagulant hirudin is found in:   
(a) In Snake
(b) In Lizard
(c) In Leech
(d) In Scorpion

Question. GEAC stands for     
(a) Genome Engineering Action Committee
(b) Ground Environment Action Committee
(c) Genetic Engineering Approval Committee
(d) Genetic and Environment Approval Committee.

Question. First Transjenic crop was :     
(a) Cotton
(b) Alsi
(c) Pea
(d) Tobacco     

 Question. How many varieties of rice has been estimated to be present in India?
(a) 2,000
(b) 20,000
(c) 200,000
(d) 2,000,000

Question. Bt toxins are   
(a) intracellular lipids 18.
(b) intracellular crystalline proteins
(c) extracellular crystalline proteins
(d) intracellular polysaccharides

Question. Bt cotton variety that was developed by the  introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to 
(a) insect pests
(b) fungal diseases
(c) plant nematodes
(d) insect predators.

Question. The first clinical gene therapy was given in 1990 to a 4 years old girl with enzyme deficiency of     
(a) Adenosine deaminase (ADA)
(b) Tyrosine oxidase
(c) Monamine oxidase
(d) Glutamate dehydrogenase

Question. Which part of the tobacco plant is infected by Meloidogyne incognita?
(a) Stem
(b) Root
(c) Flower
(d) Leaf

Question. The first transgenic crop was     
(a) tobacco
(b) cotton
(c) pea
(d) flax.

Question. Bacillus thruingiensis (Bt) strains have been used for designing noble: 
(a) Biofertilizers
(b) Biometallurgical techniques
(c) Biomineralization processes
(d) Bioinsecticidal plants

Question. A transgenic food crop which may help in solving the problem of night blindness in developing countries is
(a) golden rice
(b) Bt soyabean
(c) flavr – savr tomato
(d) starlink maize

Question. The two polypeptides of human insulin are linked together by   
(a) covalent bond
(b) disulphide bridges
(c) hydrogen bonds
(d) phosphodiester bond.

Question . Recombinant DNA technology discovered by:   
(a) Hargobind Khoorana
(b) James D. Watsan
(c) S. Kohen and H. Boer
(d) Sulton and Boveri

Question. The process of RNA interference (RNAi) has been used in the development of plants resistant to   
(a) nematodes
(b) fungi
(c) viruses
(d) insects.

Question. In RNAi, the genes are silenced using         
(a) dsRNA
(b) ssDNA
(c) ssRNA
(d) dsDNA.

Question. The plasmid of which one of the following has ‘nif’ gene in it?             
(a) Rhizobium
(b) Agrobacterium tumefaciens
(c) Bacillus thuringiensis
(d) Salmonella typhimurium

Question. A ‘new’ variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to             
(a) Co-667
(b) Sharbati Sonora
(c) Lerma Rojo
(d) Basmati.

Question. Which of the following genes do not occur naturally in living organisms?               
(a) Bt genes
(b) RNAi genes
(c) Cry genes
(d) Endogenous cytoplasmic defense genes

Question. The introduction of T-DNA into plants involves             
(a) exposing the plants to cold for a brief period
(b) allowing the plant roots to stand in water
(c) infection of the plant by Agrobacterium tumefaciens
(d) altering the pH of the soil, then heatshocking the plants.

Question. Which kind of therapy was given in 1990 to a four-year-old girl with adenosine deaminase (ADA) deficiency?             
(a) Gene therapy
(b) Chemotherapy
(c) Immunotherapy
(d) Radiation therapy

Question. Identify the incorrect statement regarding Bt cotton.           
(a) Dried spores of Bacillus thuringiensis are sprayed on its vulnerable leaves.
(b) It releases toxin that causes swelling in the gut of insect that ingest its leaves.
(c) It is a transgenic plant.
(d) It can produce toxin due to gene introduced in it by transgenesis.

Question. Select the wrong statement.               
(a) Human insulin is being commercially produced from a transgenic species of Escherichia coli.                
(b) Bt toxin genes cryIAc control the corn borer.
(c) Human protein, a-1-antitrypsin is used to treat emphysema.
(d) The first transgenic cow, Rosie, produced alpha lactalbumin, enriched milk.

Question. Bt brinjal is an example of transgenic crops. In this, Bt refers to           
(a) Bacillus tuberculosis
(b) biotechnology
(c) b-carotene
(d) Bacillus thuringiensis.

Question. Transgenic animals are extensively used for all of the following procedures except             
(a) bioremediation
(b) chemical safety
(c) vaccine safety
(d) toxicity test.

Question. The polio vaccine was tested on :
(a) Mice
(b) Monkey
(c) Rabbit
(d) Dog

Question. A pro-toxin is
(a) a primitive toxin
(b) a denatured toxin
(c) toxin produced by protozoa
(d) inactive toxin

Question. Transgenic animals are
(a) Animals whose DNA is manipulated to possess and express an extra (foreign) gene.
(b) Animals whose RNA is manipulated to possess and express an extra (foreign) gene.
(c) Animals whose both DNA and RNA are manipulated to possess and express an extra (foreign) gene.
(d) None of the above. 

Question. Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves because
(a) bacteria are resistant to the toxin.
(b) toxin is immature.
(c) toxin is inactive.
(d) bacteria enclose toxin in a special sac. 

Question. Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services.
(a) Bio-safety committee
(b) Indian council of Agricultural Research
(c) Genetic Engineering Approval committee
(d) Research Committee on Genetic Manipulation. 

Question. A probe which is a molecule used to locate specific sequences in a mixture of DNA or RNA molecules could be
(a) a single stranded RNA
(b) a single stranded DNA
(c) either RNA or DNA
(d) can be ss-DNA but not ss RNA

Question. The site of production of ADA in the body is
(a) bone marrow
(b) lymphocytes
(c) blood plasma
(d) monocytes 

We hope you liked MCQ Class 12 Biology Chapter 12 Biotechnology and its Application with answers provided above. Incase you have any questions please post them in the comments section below and our Biology teachers will provide a response.