Class 12 VBQs Biology Biotechnology and its Application

MULTIPLE CHOICE TYPE QUESTIONS

Question. The discovery that led to the development of first antibiotic was made by
(a) Jenner
(b) Pasteur
(c) Fleming
(d) Waksman

Question. Genetically engineered bacteria have been used in commercial production of
(a) Thyroxine
(b) Testosterone
(c) Human insulin
(d) Melatonin

Question. Hepatitis B vaccine is
(a) First generation vaccine
(b) Second generation vaccine
(c) Third generation vaccine
(d) Interferon

Question. Antigens are present
(a) Inside nucleus
(b) Inside cytoplasm
(c) On cell surface
(d) Inside lysosomes

Question. Biopiracy is related to the following:
(a) Traditional knowledge
(b) Biomolecules and Bioresources, Isolation of gene from Bioresources
(c) Bioresources
(d) All of the above

Question. Desired properties in plants and animals are possible by which reproduction.
(a) Genetic Engineering
(b) Chromosomal Engineering
(c) Ekabana technology
(d) Bonsai technology

Question. First Transgenic crop was
(a) Cotton
(b) poppy
(c) Pea
(d) Tobacco

Question. Transgenic Golden rice has highly rich in
(a) Lysine
(b) Methionine
(c) Glutin
(d) Vitamin-A

Question. Somatic hybridization can be done
(a) By Protoplast fusion
(b) By Haploid anther
(c) By cell culture
(d) By Pollen culture

Question. The Ti plasmid used in genetic engineering is obtained from:
(a) Bacillus thuringiensis
(b) E. Coli
(c) Agrobacterium hizogenes
(d) Agrobacterium tumefaciens

Question. A technology which has found immense use in solving cases of disputed parentage is
(a) Polymerase chain reaction
(b) DNA finger printing
(c) None of these
(d) Both (a) and (b)

Question. The two polypeptide chains of insulin are linked by
a. Phosphodiester bonds
b. Covalent bonds
c. Disulphide bonds
d. Hydrogen bonds

Question. Match the 0rganism with its use in Biotechnology
a. Bacillus thuringiensis               i-cloning vector
b. Thermus aquaticus                  ii-construction of first rDNA molecule
c. Agrobacterium tumiefaciens     iii-DNA polymerase
d. Salmonella typhimurium          iv-Cry proteins
    a  b  c  d
A  iv  iii  i  ii
B  iii  ii  iv  i
C  iii  iv  i  ii
D  ii  iv  iii  i

Question. RNAi is used for which purpose in the field of biotechnology
a. To develop a plant tolerant to abiotic stress
b. To develop a pest resistant plant against a nematode
c. To reduce post-harvest losses
d. To increase the mineral usage by plants

Question. Now a days it’s possible to detect the mutated gene causing cancer by allowing the radioactive probe to hybridise its complementary DNA in a clone of cells followed by its detection using autoradiography because
a. Mutated gene does not appear on a photographic plate as probe has no complementarity with it
b. Mutated gene does not appear on a photographic plate as probe has complementarity with it
c. Mutated gene only partially appears
d. Mutated gene appears clearly & completely on the photographic plate

Question. With regard to insulin choose the correct options
a. C-peptide is not present in mature insulin
b. The insulin produced by rDNA technology has C-peptide
c. Proinsulin has C-peptide
d. A & B peptide are connected by Disulphide bridges
choose the correct option from those given below
a. Choices a, c & d
b. Choices a & d
c. Choices b & d
d. Choices b & c

Question. @1-antitrypsin is
a. An antacid
b. An enzyme
c. Used to treat arthritis
d. Used to treat emphysema.

Question. Which among the following is based on antigen- antibody interaction?
a. PCR
b. Electrophoresis
c. ELISA
d. All of these.

Question. Choose the correct option regarding a retrovirus
a. An RNA virus that can synthesise DNA during infection
b. A DNA virus that can synthesise RNA during infection
c. A ssDNA virus
d. A dsRNA virus

Question. Cry I Ab controls
a. Corn borer
b. Stem borer
c. Root borer
d. Cotton bollworm

Question. Indicator of water pollution is
a. Ulothrix
b. Chlorella
c. E.coli
d. Nelumbium

Question. Plasmids are used as cloning vectors for which of the following reasons
a. Can be multiplied in a culture
b. Capable of self-replication in bacterial cells
c. Can be multiplied in laboratories
d. Replicates freely outside bacterial cells

Question. Choose the incorrect option with reference to a GMC
a. These are intolerant to abiotic stress
b. These do not rely on chemical pesticides
c. These reduce post-harvest losses
d. These have enhanced nutritional value

Question. The site of production of ADA in human body is
a. Bone marrow
b. RBC
c. Lymphocytes
d. Plasma

Question. Which of the following is the improved feature of transgenic Basmati rice?
a. Resistant to insects & diseases
b. Does not require chemical fertilizers
c. Give good yield & rich in Vitamin A
d. Does not need pesticides / herbicides

ASSERTION-REASON TYPE QUESTION
Questions consist of two statements – Assertion (A) and Reason (R).
Answer these questions selecting the appropriate option given below:
A. Both A and R are true and R is the correct explanation of A
B. Both A and R are true and R is not the correct explanation of A
C. A is true but R is false
D. A is False but R is true

Question. Assertion: Biotechnology is providing the best catalyst in the form of improved microbes or pure Enzyme.
Reason: Biotechnology is creating optimal conditions through engineering for a catalyst to act.

Question. Assertion: Genetic modification has made crops more tolerant to abiotic stresses of the environment.
Reason: Genetic modifications in crop has increased the post-harvest losses.

Question. Assertion: Proteins encoded by genes Cry IAc and Cry IIAb control the cotton ball worm.
Reason: The proteins Cry IAb Controls corn borer.

Question. Assertion: SCID patient is devoid of T lymphocytes and B lymphocytes.
Reason: There is deficiency of Adenosine deaminase (ADA) enzyme.

Question. Assertion: PCR is used to detect AIDS infection to human being.
Reason: Very low concentration of viral DNA or RNA can be amplified by PCR.

Question. Assertion: Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans and coleopterans.
Reason: Bt toxin protein exist as inactive protoxin but once an insect ingest the inactive toxin it is converted in to an active form of toxin due to acidic pH of the gut which dissolves the crystals.

Question. Assertion: RNAi takes place in all eukaryotes as a method of cellular defense.
Reason: RNAi involves silencing of specific mRNA due to complimentary ds RNA molecule that binds to and prevent translation of mRNA.

Question. Assertion: Fully functional and mature insulin consist of three polypeptide chains: chain A, chain B and chain C peptide.
Reason: Insulin produced by Eli Lilly company has chains A and B only and they are combined by disulphide bonds.

Question. Assertion: ADA deficiency can be treated by Gene therapy.
Reason: ADA gene isolated from bone marrow cells is introduced in to patients cells at early embryonic stages.

Question. Assertion: Milk produced by the first transgenic cow, Rosie was nutritionally more balanced and superior 
Reason: Milk contained the human alpha lactalbumin.

Question. Assertion: ADA enzyme is crucial for the immune system to function. 
Reason: ADA deficiency is caused due to malnutrition.

Question. Assertion: ELISA is widely used for the detection of infectious diseases like AIDS.
Reason: ELISA is based on the principle of antigen antibody interaction.

Question. Assertion: Nematode Meloidegyne incognitia infects the leaves of tobacco plants and causes great reduction in yield.
Reason: A novel strategy was adopted to prevent the infestation of this nematode was based on the process of RNA interference.

Question. Assertion: Government has set up organizations like GEAC which will make decisions regarding validity of GM research and the safety of introducing GM organisms for public services. 
Reason: Genetic modification of organisms can have unpredictable results when such organisms are introduced in to ecosystem.

Question. Assertion: Genetic modification of crops has reduced reliance on chemical pesticides   
Reason: Genetic modification has decreased efficiency of mineral usage by plants

Question. Assertion: Recombinant therapeutics are safe and more effective than similar products isolated from non-human sources. 
Reason: The recombinant therapeutics do not induce unwanted immunological responses

Question. Assertion: Bacillus thuringiensis produce protein crystals that can kill only lepidopteran insects 
Reason: Protein crystals which do not kill the Bacillus as it exists as inactive protoxin

Question. Assertion: Animals that have had their DNA manipulated to possess and express an extra gene are known as transgenic animals.  
Reason: Transgenic mice are not being used to test the safety of the polio vaccine

Question. Assertion: The ADA gene gives instruction for producing the enzyme adenosine deaminase. 
Reason: Throughout the body this enzyme is found but is most active in lymphocytes.

Question. Assertion: Biotechnology produces transgenic micro-organisms that act as micro factories for Proteins. 
Reason: To produce proteins of human use like insulin transgenic microorganisms can be Developed.